Penicillin acylase‐catalyzed protection and deprotection of amino groups as a promising approach in enzymatic peptide synthesis

FEBS Letters

Didžiapetris, Remigijus¹  Drabnig, Barbara²  S̆vedas, Vytas¹  Jakubke, Hans-Dieter²  Schellenberger, Volker² 

[1] A.N. Belozersky Institute of Physio-Chemical Biology, Moscow Slate University,Moscow 119899, USSR;Department of Biochemistry, Biosciences Division, Leipzig University, 0-7010, Leipzig, Germany

关键词: Penicillin acylase (E. coli);    Enzymatic peptide synthesis. Enzymatic amino group protection and deprotection: Papain;    α-Chymotrypsin;    Leucineenkephalin;    CT;    α-chymotrypsin;    OBut;    t-butyl ester;    OMe;    methyl ester;    PA;    penicillin acylase;    P;    papain;    PhAc;    phenylacetyl;   

DOI  :  10.1016/0014-5793(91)80009-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Penicillin acylase from E. coli is able to catalyze both the introduction and the removal of the phenylacetyl group. We have established that phenylacetyl derivatives of amino acids and peptides can be used in protease-catalyzed peptide synthesis. Here the synthesis of leucine-enkephalin using enzymes for N-terminal amino group protection, peptide bond formation and deprotection is described.

【 授权许可】

Unknown