Thiolation of preformed liposomes with iminothiolane

FEBS Letters

Bogdanov, Alexander A.¹  Niedermann, Gabriele²  Torchilin, Vladimir P.¹  Lasch, Jürgen² 

[1] USSR Cardiology Research Center, Academy of Medical Sciences, 3rd Cherepkovskaya 15 A, 121 552 Moscow, USSR;Institute of Biochemistry, Martin-Luther-University Halle-Wittenberg, Hollystrasse 1, PSF 184, DDR-4020 Halle/S., GDR

关键词: Thiol-liposome;    2-Iminothiolane;    Membrane thiolation;    α Immobilization;    α-Chymotrypsin;    DTNBS;    5;    5′-dithiobis(2-nitrobenzoic acid);    SPDP;    N-hydroxysuccinimidyl-3-(2-pyridyldithio)propionate;    PDP-;    N-3-(2-pyridyldithio)propionyl-;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    DPPE;    dipalmitoylphosphatidylethanolamine;    ATEE;    acetyltyrosine ethyl ester;    α-CT;    α-chymotrypsin;    DTE;    dithiothreitol;    TNBS;    2;    4;    6-trinitrobenzene sulfonic acid;    SUV;    small unilamellar vesicle;   

DOI  :  10.1016/0014-5793(87)80004-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Preformed phosphatidylethanolamine-containing liposomes were thiolated with 2-iminothiolane (Traut's reagent) and subsequently activated by mixed disulfide formation with 5,5′-dithiobis(2-nitrobenzoic acid). Up to 65% of amino groups of the outer liposomal lamella, corresponding to 230 SH-groups per vesicle, were modified. Covalent attachment of thiolated α-chymotrypsin to these thiol-liposomes via S-S bridges yielded a protein/lipid ratio of 3.6 × 10⁻⁴ mol enzyme/mol lipid.

【 授权许可】

Unknown