Fixation of a highly reactive form of α‐chymotrypsin by micellar matrix

FEBS Letters

Bogdanova, N.G.²  Klyachko, N.L.²  Levashov, A.V.²  Martinek, K.¹ 

[1] Institute of Organic Chemistry and Biochemistry, Prague, Czechoslovakia;Department of Chemistry, M.V. Lomonosov Moscow State University Moscow, USSR

关键词: α-Chymotrypsin;    Conformation;    Solvated reversed micelle;    Substrate specificity;   

DOI  :  10.1016/0014-5793(90)81017-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using reversed micelles of surfactants solvated by water-organic cosolvent mixtures as a matrix for enzyme entrapping, it is possible to fix the highly reactive α-chymotrypsin form. The reactivity of α-chymotrypsin towards nonspecific substrates increases to the extent comparable with that observed in reactions involving specific substrates.

【 授权许可】

Unknown