Inverse regulation of glucose transporter Glut4 and G‐protein Gs mRNA expression in cardiac myocytes from insulin resistant rats

FEBS Letters

Russ, Martina¹  Reinauer, Hans¹  Eckel, Jürgen¹  Petersen, Simone¹ 

[1] Laboratory of Molecular Cardiology, Diabetes Research Institute, 4000 Düsseldorf 1, Germany

关键词: Isolated cardiac myocyte;    Diabetes;    Insulin resistance;    Glucose transporter;    Guanine nucleotide-binding protein;    Messenger ribonucleic acid;   

DOI  :  10.1016/0014-5793(91)80927-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The present study examined the mRNA levels of glucose transporter Glut4 and G-protein G_s α-subunit in isolated ventricular myocytes from lean and genetically obese (fa/fa) Zucker rats and streptozotocin-diabetic rats. In obese animals the amount of transcripts coding for Glut4 increased to 122±6% of lean controls, whereas the mRNA coding for G_s α-subunit decreased by 42±12%. An unaltered level of G_s mRNA was observed in insulin deficient rats. When cardiomyocytes from normal rats were treated with insulin, the Glut4 transcript level increased by 48±5%, whereas the G_s mRNA level decreased by 55±8%. The findings suggest that insulin may act as a potential regulator of Glut4 and G_s mRNA expression in the cardiac cell.

【 授权许可】

Unknown