The complete amino acid sequence of bovine cathepsin S and a partial sequence of bovine cathepsin L

FEBS Letters

Čolić, A.²  Ogrinc, T.¹  Podobnik, M.²  Turk, V.¹  Ritonja, A.¹  Dolenc, I.¹ 

[1] Department of Biochemistry, Jozžef Stefan Institute, Jamova 39, 61000 Ljubljana, Yugoslavia;Centre for Genetic Engineering and Biotechnology, Filipa Kljajica 12, 71000 Sarajevo, Yugoslavia

关键词: Cathepsin S;    Cathepsin L;    Cysteine proteinase;    Amino acid sequence;   

DOI  :  10.1016/0014-5793(91)80620-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequence of bovine spleen cathepsin S has been determined. The single-chain protein 217 residues and has a M _r of 23 682. The primary structure was determined by sequencing of native protein and the peptides obtained by proteolytic cleavage with β-trypsin, papaya proteinase IV and by chemical cleavage with cyanogen bromide. Comparison of the amino terminal sequences of the heavy and the light chain of bovine cathepsin L with that of bovine cathepsin S clearly indicates that the enzymes are structurally different.

【 授权许可】

Unknown