Translocation of the α‐ and β‐isoforms of protein kinase C following activation of human T‐lymphocytes

FEBS Letters

Jondal, Mikael¹  Fredholm, Bertil B.¹  Kvanta, Anders² 

[1] Department of Immunology, Karolinska Institutet, Box 60400, S-104 01 Stockholm, Sweden;Department of Pharmacology, Karolinska Institutet, Box 60400, S-104 01 Stockholm, Sweden

关键词: Translocation;    Isoform;    Protein kinase C;    T-lymphocyte;    PMA;    phorbol 12-myristate 13-acetate;    PHA;    phytohemagglutinin;    Con A;    concanavalin A;    PKC;    protein kinase C;   

DOI  :  10.1016/0014-5793(91)80618-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have analyzed how activation of human Jurkat T-cells by the mitogenic lectin, concanavalin A (Con A), may affect the cellular distribution of the α- and β-isoforms of protein kinase C (PKC) in T-cells. In non-stimulated cells almost all of the α- and β-PKC was localized to the cytoplasmic compartment. Stimulation with Con A caused a transient translocation of both α- and β-PKC from the cytoplasm to the cell membrane. The α- isoform appeared to be translocated to a somewhat greater extent and for a longer period of time that the β-form. Translocation was maximal between 1 and 5 min for both of the isoforms. 30 min after stimulation, β-PKC had returned to basal levels, whereas a substantial amount of α-PKC remained associated with the particulate fraction. We conclude that activation of human T-cells causes the translocation of at least two different isoforms of PKC, α-PKC and β-PKC.

【 授权许可】

Unknown