Characterization and differential expression of protein kinase C isoforms in PC12 cells Differentiation parallels an increase in PKC beta11

FEBS Letters

Lloyd, Elizabeth D.¹  Ewald, Sandra J.²  Olivier, Andree³  Parker, Peter J.³  Soh, Yunjo⁴  Wooten, Marie W.¹  Seibenhener, M.Lamar¹  White, Kimberly R.¹ 

[1] Department of Zoology, Alabama Agricultural Experiment Station, Auburn University, Auburn, AL 36830, USA;Poultry Science and Pathobiology, Alabama Agricultural Experiment Station, Auburn University, Auburn, AL 36830, USA;ICRF, Protein Phosphorylation Laboratory, London, England;Chemistry, Alabama Agricultural Experiment Station, Auburn University, Auburn, AL 36830, USA

关键词: Protein kinase C;    Isoform;    Differentiation;    Nerve growth factor. PC12;   

DOI  :  10.1016/0014-5793(92)80025-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nerve growth factor (NGF) treatment of PC12 cells induced a 2.8-fold increase in protein kinase C activity concomitant with differentiation and acquisition or neurites. PKC protein isoforms were separated by sequential chromatography on DEAE-Sephacel/hydroxylapatite. A broad peak or PKC activity eluted which corresponded to the alpha PKC isoform. In control cells, message for all six PKC isoforms was detected and expressed as epsilon>zeta=gamma>delta>beta>alpha. Western blot or whole cell lysates revealed a large increase in the beta₁₁, while slight changes were observed for the other five PKC isoforms during treatment (12-14 days) with NGF (50 ng/ml). In parallel, coordinate changes in the expression of the individual transcripts for the six isoforms occurred during NGF treatment. Induction and accumulation of PKC beta₁₁ may play a role in maintenance or neuronal morphology.

【 授权许可】

Unknown