Translocation of diacylglycerol kinase α to the nuclear matrix of rat thymocytes and peripheral T‐lymphocytes

FEBS Letters

Kanoh, Hideo¹  Imai, Shin-ichi¹  Sakane, Fumio¹  Wada, Ikuo¹  Kai, Masahiro¹ 

[1] Department of Biochemistry, Sapporo Medical University School of Medicine, South-1, West-17, Sapporo 060, Japan

关键词: Diacylglycerol kinase;    α-isozyme;    T-lymphocyte;    rat;    Translocation;    Nuclear matrix;   

DOI  :  10.1016/0014-5793(96)00857-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cytosolic α-diacylglycerol kinase (DGK) was translocated to and tightly associated with the nuclear matrix when rat thymocytes and peripheral T-lymphocytes were stimulated with concanavalin A or anti-T-cell receptor antibody. This translocation occurred rather slowly and was completed in 3–4 h after cell stimulation. We also detected significant accumulation of nuclear phosphatidic acid interpreted as being formed by the translocated enzyme. The enzyme translocation is not directly linked to phosphoinositide turnover and protein phosphorylation, since phorbol myristate acetate and calcium ionophore did not affect the cellular DGKα and since we detected no covalent modification of the enzyme molecule. Although the mechanisms underlying the enzyme translocation remain unknown, our results indicate that DGKα participates in nuclear phospholipid metabolism occurring at the intermediate stage of lymphocyte activation.

【 授权许可】

Unknown