Defect in phorbol acetate‐induced translocation of diacylglycerol kinase in erbB‐transformed fibroblast cells

FEBS Letters

Kato, Miwako¹  Takenawa, Tadaomi¹  Kawai, Sadaaki² 

[1] Department of Pharmacology, Tokyo Metropolitan Institute of Gerontology, Sakae-cho, Itabashi-ku, Tokyo 173, Japan;Department of Tumor Virus Research, Institute of Medical Science, The University of Tokyo, Shirokane-dai, Minato-ku, Tokyo 108, Japan

关键词: Diacylglycerol kinase;    Translocation;    Oncogene;    erbB;    Oncogene;    src;   

DOI  :  10.1016/0014-5793(89)81345-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We here show that tetradecanoyl phorbol acetate (TPA) and 1-oleoyl 2-acetyl glycerol (OAG) cause the translocation of diacylglycerol (DG) kinase from the cytosol to the membrane fractions in chick embryo fibroblast (CEF) cells. However, this translocation is not marked in erbB-transformed chick embryo fibroblast (GEV) cells. The activities of phosphatidylinositol (PI) and phosphatidylinositol 4-phosphate (PIP) kinases in membrane fractions are not altered by TPA treatment in either CEF or GEV cells. Such reduced translocation of DG kinase by TPA is also observed in src-transformed cells, but not in myc-transformed cells. These results suggest that the defect in DG kinase translocation may result in failure to suppress the overactivation of protein kinase C in erbB-2 and src-transformed cells, which may lead to cell growth and transformation.

【 授权许可】

Unknown