Differential synthesis by cultured atrial and ventricular rat cardiac myocytes of myosin light chain isoforms

FEBS Letters

Milting, Hendrik³  Zippel, Monika²  Brand, Thomas¹  Jockusch, Harald² 

[1] MPI für Physiologische und Klinische Forschung, Abteilung Experimentelle Kardiologie, Benekestr. 2, D(W)-6350 Bad Nauheim, Germany;Developmental Biology Unit, W7, University of Bielefeld, POB 8640, D(W)-4800 Bielefeld 1, Germany;Institut für Physiologische Chemie der Ruhr-Universität, Universitätsstr. 150, D(W)-4630 Bochum, Germany

关键词: Cell culture;    2D electrophoresis;    Neonatal rat;    Protein synthesis;   

DOI  :  10.1016/0014-5793(91)80610-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dissociated cells from neonatal rat atria and ventricles were cultured in monolayers for 3 days. Newly synthesized ³⁵S-methionine labeled myosin light chain isoforms ALC-1, ALC-2 (atrial) and VLC-1, VLC-2 (ventricular) were identified on 2D gels, and their pattern of synthesis was compared to that of myocard fragments immediately after explantation. ALCs were synthesized in 5- to 10-fold excess over VLCs by atrial cultures, whereas the converse was true for ventricular cultures, with two exceptions: one third of the LC-1 synthesized by ventricular fragments was ALC-1, and dissociated atrial cells synthesized very little LC-2 of either isoform. The former finding corresponds to the relatively high proportion of ALC-1 in neonatal ventricular tissue. We conclude that the regional programme of LC isoform expression is basically retained after tissue explantation and even after dissociation and culturing of cardiac myocytes.

【 授权许可】

Unknown