The three‐dimensional structure of proteasomes from Thermoplasma acidophilum as determined by electron microscopy using random conical tilting

FEBS Letters

Baumeister, Wolfgang¹  Pfeifer, Günter¹  Hegerl, Reiner¹  Pühler, Gabriela¹  Dahlmann, Burkhardt² 

[1] Max-Planck-Institut für Biochemie, D-8033 Martinsried, Germany;Diabetes-Forschungsinstitut an der Universität Düsseldorf, D-4000 Düsseldorf, Germany

关键词: Proteasome;    Archaebacterium;    Thermoplasma acidophilum;    Electron microscopy;    Reconstruction 3-D;    Conical tilting;    random;   

DOI  :  10.1016/0014-5793(91)80567-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The three-dimensional structure of proteasomes from the archaebacterium Thermoplasma acidophilum has been determined to a resolution of approximately 2 nm from electron micrographs of negatively stained preparations using the method of random conical tilting. The particles turn out to be essentially cylinder-shaped barrels, 15 nm long and 11 nm wide, enclosing a tripartite inner compartiment. An account is given of some of the present limitations which prevent to attain a higher resolution and possible ways to overcome these limitations are indicated.

【 授权许可】

Unknown