Cloning and sequencing of the gene encoding the large (α‐) subunit of the proteasome from Thermoplasma acidophilum

FEBS Letters

Lottspeich, Friedrich²  Baumeister, Wolfgang²  Zwickl, Peter²  Dahlmann, Burkhardt¹ 

[1] Diabetes-Forschungsinstitut an der Univesität Düsseldorf, D-4000 Düsseldorf, Germany;Max-Planck-Institut für Biochemie, D-8033 Martinsried, Germany

关键词: Proteasome;    Prosome;    Multicatalytic proteinase;    Archaebacterium;    Thermoplasma acidophilum;    crl;    mutation encoding cycloheximide and temperature sensitive lethality;    HPLC;    high-performance liquid chromatography;    sell +;    wildtype allele of sell;    SCLI-1;    mutant allele of sell;    dominant suppressor of crl3 temperature-sensitive lethality;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    TFA;    trifluoroacetic acid;    YC;    yeast component of the proteasome;   

DOI  :  10.1016/0014-5793(91)80120-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene encoding the α-subunit of the proteasome from Thermoplasma acidophilum was cloned and sequenced. The gene encodes for a polypeptide with 233 amino acid and reduces a calculated molecular weight of 25870. Sequence similarity of the α-subunit with the Saccharomyces cerevislae wild-type suppressor gene sell* encoded polypeptide, which is probably identical with the subunit YC7-α of the yeast proteasome, lends support to a putative role of proteasomes in the regulation of gene expression. The significant sequence similarity to the various subunits of eukaryotic proteasomes make it likely that proteasomal proteins are encoded by one gene family of ancient origin.

【 授权许可】

Unknown