Alternative splicing generates two isoforms of the α2 subunit of the inhibitory glycine receptor

FEBS Letters

Betz, H.²  Maulet, Y.¹  Kuhse, J.¹  Schmieden, V.¹  Kuryatov, A.¹  Malosio, M.L.¹ 

[1] Zentrum für Molekulare Biologie Heidelberg, Universität Heidelberg, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany;Max-Planck Institut für Hirnforschung, Deutschordenstrasse 46, D-6000 Frankfurt 1, Germany

关键词: Glycine receptor;    Alternative splicing;    Receptor heterogeneity;    Brain development;   

DOI  :  10.1016/0014-5793(91)80557-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR α2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced protein sequences (α2A and α2B) exhibit 99% identity with the previously characterized human α2 subunit. In situ hybridization revealed expression of both α2A und α2B mRNAs in the prenatal rat brain, suggesting that these variant proteins may have a role in synaptogenesis. Heterologous expression in Xenopus oocytes showed that the more abundantly expressed α2A subunit forms strychnine-sensitive ion channels which resemble human α2 subunit GlyRs in their electrophysiological properties.

【 授权许可】

Unknown