Two forms of the GABAA receptor distinguished by anion‐exchange chromatography

FEBS Letters

Neale, Joseph H.¹  Moffett, John R.¹  Namboodiri, Aryan¹ 

[1] Department of Biology, Georgetown University, Washington, DC 20057, USA

关键词: Aminobutyric acid receptor;    γ-;    Benzodiazepine;    Receptor heterogeneity;    Anion-exchange HPLC;    CL 218872;   

DOI  :  10.1016/0014-5793(89)81245-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The GABA_A receptor complex was solubilized from rat brain membranes in Triton X-100, enriched by 1012-S affinity chromatography, and subjected to DEAE anion-exchange chromatography. Two forms were distinguished by their differential elution during this HPLC with a KCl gradient. They displayed similar [³H]muscimol- and [³H]flunitrazepam-binding characteristics, as well as [³H]flunitrazepam-binding inhibition by CL 218872. Rechromatography of these distinct ionic forms indicated that they were not in dynamic equilibrium during chromatography. Resolution of these two pharmacologically similar populations of GABA_A receptor by anion-exchange HPLC suggests that they differ in charge densities, a condition which may reflect differing glycosylation or phosporylation states of the complex.

【 授权许可】

Unknown