Effective method for obtaining long nucleotide chains on partially complementary templates Processed bovine γ‐interferon gene obtained from human γ‐interferon gene

FEBS Letters

Azhikina, T.L.¹  Rostapshov, V.M.¹  Chernov, I.P.¹  Sverdlov, E.D.¹  Borodin, A.M.¹ 

[1] M.M. Shemyakin Institute of Bioorganic Chemistry, ul.Miklukho-Maklaya, 16/10,117871 GSP Moscow V-437, USSR

关键词: Site-directed mutagenesis;    Interferon;    γ-;    Sequence homology;    Gene variant;    (Bovine);   

DOI  :  10.1016/0014-5793(89)80663-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The method of obtaining the bovine γ-interferon gene by means of simultaneous multidirected mutagenesis of the human γ-interferon gene is presented. The first strand of the bovine γ-interferon gene was obtained by ligation of synthetic oligonucleotides, using the cDNA of human γ-interferon, cloned in the single-stranded phage M13mpl9 as a template. The second strand was synthesized using a large fragment of E. coli DNA-polymerase 1. The double-stranded gene was then treated by restriction nucleases and cloned in a pUC- 18 derived vector. The primary structure was confirmed by sequencing.

【 授权许可】

Unknown