Identification of the α3‐subunit in the GABAA receptor purified from bovine brain

FEBS Letters

Duggan, Michael J.¹  Casalotti, Stefano O.¹  Stephenson, F.Anne¹ 

[1] MRC Molecular Neurobiology Unit, MRC Centre, Hills Road, Cambridge CB2 2QH, England

关键词: Benzodiazepine;    Cl− channel;    Antibody;    Receptor;    Aminobutyric acid;    γ-;    GABA;    γ-aminobutyric acid;    KLH;    keyhole limpet haemocyanin;    ELISA;    enzyme-linked immunoadsorbent assay;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    TBPS;    t-butylbicyclophosphorothionate;   

DOI  :  10.1016/0014-5793(89)80161-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyclonal antibodies have been raised to synthetic amino acid sequences of the bovine GABA_A receptor α₁ and α₃ subunits. Anti-α₁ subunit antibodies recognise a polypeptide of 53 kDa whereas anti-α₁ subunit antibodies recognise a polypeptide of 59–60 kDa, in Western blots of GABA_A receptor purified from adult bovine cerebral cortex, cerebellum and 12-day calf cerebral cortex.

【 授权许可】

Unknown