Heterogeneous distribution of nuclear triiodothyronine receptors in liver and preadipose cells as evidenced by their reactivity to antibodies against different protein products of erb A oncogenes

FEBS Letters

Savary, J.²  Ghysdael, J.¹  Ghiringhelli, O.²  Teboul, M.²  Bismuth, J.²  Torresani, J.² 

[1] INSERM Unité 186, Institut Pasteur, Lille, France;INSERM U 38, CNRS URA 178, Laboratoire de Biochimie Médicale, Faculté de Médecine, Marseille Institut Pasteur, Lille, France

关键词: Thyroid hormone;    Receptor;    Oncogene;    erb A;    Antibody;    (Liver;    Preadipocyte);   

DOI  :  10.1016/0014-5793(89)80732-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyclonal antibodies raised in rabbits against bacterially produced peptides in the C-terminal region of v-erb A or human c-erb A_α oncogenes recognize the nuclear triiodothyronine (T3) receptors in the T3-sensitive Ob 17 mouse preadipocyte cell line and not in mouse or rat liver. The results confirm the existence of different T3 receptors in different tissues. The results also suggest a heterogeneous receptor distribution within the preadipose cell line, with a predominance of c-erb A_α-type species. Antibodies raised against domain 149–227, but not against domain 245–325, impair T3 binding, suggesting a role for this domain in ligand binding.

【 授权许可】

Unknown