Promiscuity of GABAA‐receptor β3 subunits as demonstrated by their presence in α1, α2 and α3 subunit‐containing receptor subpopulations

FEBS Letters

Pollard, Simon¹  Duggan, Michael J.¹  Stephenson, F.Anne¹ 

[1] Department of Pharmaceutical Chemistry, School of Pharmacy, 29/39 Brunswick Square, London WC1N 1AX, UK

关键词: GABAA;    Receptor;    Benzodiazepine;    Antibody;    Immunoaffinity;    Purification;    ELISA;    enzyme linked immunoadsorbent assay;    GABA;    γ-aminobutyric acid;    KLH;    keyhole limpet haemocyanin;    SDS-PAGE;    sodium dodecyl sulphate polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(91)81390-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyclonal antibodies were raised in rabbits against the GABA_A-receptor β3 subunit peptide sequence, KQSMPREGHGRHMDR-NH₂ coupled to keyhole limpet haemocyanin. These anti-β3 379–393 antibodies immunoprecipitated in a dose-dependent manner specific benzodiazepine agonist binding sites from Na⁺ deoxycholate extracts of bovine cerebral cortex. In immunoblots, anti-β3 379–393 antibodies recognised two species with M _fr 59 900 and M _fr 57 200 in all preparations tested, which included crude detergent-solubilised, benzodiazepine affinity chromatography-purified receptor, anti-α1 324–341 antibody, anti-Cys α2 414–424 antibody and anti-Cys α3 454–467 antibody immunoaffinity-purified GABA_A-receptor subpopulations. These results provide evidence for the ubiquity and promiscuity of the GABA_A-receptor β3 subunit.

【 授权许可】

Unknown