Molecular cloning and sequencing of genomic DNA encoding yeast vacuolar carboxypeptidase yscS

FEBS Letters

Suárez-Rendueles, Paz¹  Santiago, Gascón¹  Bordallo, Javier¹  Bordallo, Carmen¹ 

[1] Area de Bioquimica y Biologia Molecular, Departamento de Biologia Functional, Universidad de Oviedo, E-33071 Oviedo, Spain

关键词: Carboxypeptidase;    Molecular cloning;    Vacuole;    Yeast;   

DOI  :  10.1016/0014-5793(91)80546-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A Saccharomyces cerevisiae genomic DNA encoding vacuolar carboxypeptidase yscS was cloned from a yeast YEp 13 library by complementation of the previously characterized mutation epsl-1 [(1981) J. Bacteriol. 147, 418–426], by means of staining carboxypeptidase activity in yeast colonies. The nucleotide sequence of the cloned gene was determined. The open reading frame of CPS1 consists of 576 codons and therefore encodes a protein of 64961 molecular weight. A stretch of 19 residues near the N-terminus of the deduced polypeptide sequence contains characteristics common to known hydrophobic leader sequences. CPS1 was determined by DNA blot analysis to be a single copy gene located on chromosome X. The cloned fragment was used to identify a 2.1 kb mRNA. A transcriptional activation of CPS1 occurs when cells grow on a substrate of carboxypeptidase yscS as sole nitrogen source.

【 授权许可】

Unknown