AMPA and kainate‐operated channels reconstituted in artificial bilayers

FEBS Letters

Prestipino, G.²  Barnard, E.A.¹  Ambrosini, A.¹ 

[1] MRC, Molecular Neurobiology Unit, MRC Centre, Hills Road, CB2 2QH Cambridge, UK;CNR, Istituto di Cibernetica e Biofisica, Via Dodecaneso, 33-16146 Genova, Italy

关键词: Unitary non-NMDA receptor;    Reconstitution;    Ion channel;    Xenopus;    AMPA;    α-amino-3-hydroxy-5-methylisoxazole-4-propionate;    NMDA;    N-methyl-D-aspartate;    KA;    kainate;    QUIS;    quisqualate;    OG;    octylglucoside;    CNQX;    6-cyano-7-nitroquinoxaline-2;    3-dione;   

DOI  :  10.1016/0014-5793(91)80350-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Catlonic channels which can be activated by α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) and kainate play a key role in the generation of excitatory posisynaptic potentials in the brain of all vertebrates. On a protein carrying binding sites for both these ligands, affinity-purified from the Xenopus nervous system, electrical measurements have been performed to investigate its functional properties after the pure complex had been incorporated into planar lipid bilayers. Domoate, AMPA or kainate added to the reconstituted protein activated cationic channels, which were blocked by typical antagonists for this neurotransmitter system. These data suggest that the reconstituted protein is an ionotropic receptor of the unitary non-NMDA sybtype.

【 授权许可】

Unknown