The monocarboxylate carrier from rat liver mitochondria

FEBS Letters

Di Paola, Marco¹  Azzi, Angelo²  Capuano, Ferdinando¹  Papa, Sergio¹ 

[1] Institute of Medical Biochemistry and Chemistry, University of Bari, Bari, Italy;Institutefur Biochemie und Molekularbiologie der Universität Bern, Bühlstrasse 28, CH-3012 Bern, Switzerland

关键词: Monocarboxylate carrier;    Pyruvate transport;    Reconstitution;    Mitochondria;    (Rat liver);    PAGE;    polyacrylamide gel electrophoresis;    HTP;    hydroxylapatite;    LDH;    lactate dehydrogenase;    3-HBDH;    3-hydro-xybutyrate dehydrogenase;   

DOI  :  10.1016/0014-5793(90)80631-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The monocarboxylate (pyruvate) carrier was extracted from rat liver mitochondria with Triton X-100 in the presence of asolectin and partially purified by chromatography on HTP. The HTP eluate reconstituted in liposomes was shown to catalyze active pyruvate_in/acetoacetate_out, and acetoacetate_in/pyruvate_out counter-exchange. Kinetic characterization of the reconstituted pyruvate carrier was achieved by an original spectrophotometric method consisting of determination of substrate release from proteoliposomes with a coupled enzymatic assay.

【 授权许可】

Unknown