Pyruvate uptake induced by a pH jump in mesophyll chloroplasts of maize and sorghum, NADP‐malic enzyme type C4 species

FEBS Letters

Ohnishi, Jun-ichi¹  Kanai, Ryuzi¹ 

[1] Department of Biochemistry, Faculty of Science, Saitama University, Urawa 338, Japan

关键词: Pyruvate transport;    Mesophyll chloroplast;    C4 photosynthesis;    pH jump;    Proton gradient;    Zea mays;    CCCP;    carbonylcyanide-m-chlorophenylhydrazone;    MCp;    mesophyll chloroplasts;    PEP;    phosphoenolpyruvate;    [Pyr]in;    stromal concentration of pyruvate;   

DOI  :  10.1016/0014-5793(90)81134-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A sudden pH decrease (pH jump) of the medium enhanced pyruvate uptake in the dark in mesophyll chloroplasts (MCp) of Zea mays and Sorghum bicolor, NADP-malic enzyme type C₄ plants, while it was reported that a Na⁺ jump enhanced pyruvate uptake in MCp of P. miliaceum, a NAD-malic enzyme type [(1987) FEBS Lett. 219, 347]. The enhancement effect of the pH jump decayed completely in 5 min and the decay was accelerated by proton gradient-collapsing reagents. The results suggest that active pyruvate uptake into MCp of NADP-malic enzyme type C₄ species is primarily driven by the proton gradient across the envelope.

【 授权许可】

Unknown