Transient deprotonation of bacterial halorhodopsin by photoexcited base

FEBS Letters

Nachliel, E.¹  Gutman, M.¹ 

[1] Department of Biochemistry, Tel Aviv University, Faculty of Life Sciences, Tel Aviv 69978, Israel

关键词: Halorhodopsin;    Difffusion-controlled reaction;    Excited state proton transfer;    pH jump;    6-Methoxyquinoline;   

DOI  :  10.1016/0014-5793(85)80420-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The excited singlet state of 6-methoxyquinoline is basic enough to abstract a proton from water and generate OH⁻. Pulse excitation of such a solution undergoes a massive transient alkalinization. This procedure was employed to monitor the deprotonation of the Schiff base of halorhodopsin. Both the deprotonation and reprotonation of the chromophore are diffusion controlled reactions.

【 授权许可】

Unknown