Direct cleavage of a DNA fragment by a bleomycin‐oligonucleotide derivative

FEBS Letters

Godovikova, T.S.¹  Zarytova, V.F.¹  Sergeyev, D.S.¹ 

[1] Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk 630090, USSR

关键词: Reactive oligonucleotide derivative;    Antitumor antibiotic;    Bleomycin;    Direct Damage of nucleic acids;    BLM-RH;    bleomycin A5;    I;    BLM-R-pd(CCAAACA);    Ia;    pd(CCAAACA);    II;    pd(TCCTTCG);   

DOI  :  10.1016/0014-5793(91)80309-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bicomycin A₁ oligonucleotide derivative was used for direct cleavage of a DNA target. In the presence of Fe²⁺ ions and 2-mercaptoethanol, Blm-R-pd(CCAAACA) (I) damaged the target, pd(TGTTTGGCGAAGGA), with the yield of 80%, without affecting its own oligonucleotide tail. The sites of the cleavage were T^?-T^? and G^?-G^?. Unbound bleomycin A₅, damaged the G⁶-G⁷-G⁸ site. Reagent I formed more stable complementary complexes with the target than parent oligonucleotide (ΔT _m=11°C)

【 授权许可】

Unknown