期刊论文详细信息
FEBS Letters | |
Direct cleavage of a DNA fragment by a bleomycin‐oligonucleotide derivative | |
Godovikova, T.S.1  Zarytova, V.F.1  Sergeyev, D.S.1  | |
[1] Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk 630090, USSR | |
关键词: Reactive oligonucleotide derivative; Antitumor antibiotic; Bleomycin; Direct Damage of nucleic acids; BLM-RH; bleomycin A5; I; BLM-R-pd(CCAAACA); Ia; pd(CCAAACA); II; pd(TCCTTCG); | |
DOI : 10.1016/0014-5793(91)80309-Q | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Bicomycin A1 oligonucleotide derivative was used for direct cleavage of a DNA target. In the presence of Fe2+ ions and 2-mercaptoethanol, Blm-R-pd(CCAAACA) (I) damaged the target, pd(TGTTTGGCGAAGGA), with the yield of 80%, without affecting its own oligonucleotide tail. The sites of the cleavage were T?-T? and G?-G?. Unbound bleomycin A5, damaged the G6-G7-G8 site. Reagent I formed more stable complementary complexes with the target than parent oligonucleotide (ΔT m=11°C)
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020294615ZK.pdf | 355KB | download |