A Drosophila nuclear localisation signal included in an 18 amino acid fragment from the serendipity δ zinc finger protein

FEBS Letters

Noselli, Stéphane¹  Vincent, Alain¹ 

[1] Centre de Recherche de Biochemie et Génétique Cellulaire, 118 route de Narbonne, 31062 Toulouse Cedex, France

关键词: Nuclear localisation sequence;    β-Galactosidase fusion;    Finger protein;    Transgenic fly;    Drosophila melanogaster;   

DOI  :  10.1016/0014-5793(91)80229-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sequence analysis of the nuclear Drosophila serendipity δ Cys-2/His-2 finger protein indicated the presence of a short motif of positively charged amino acids, with homology of the SV40 large T and c-myc nuclear localisation signals. Using P-element mediated transformation we constructed transgenic Drosophila lines expressing β-galactosidase fusion proteins, containing (or not) an 18 residue segment of sry δ including this basic, PTKKRVK, motif. Histochemical detection of fusion proteins on dissected tissues showed that this segment of sry δ can act autonomously to drive the β-galactosidase in nuclei.

【 授权许可】

Unknown