A functional Spo0A is required for maximal aprE expression in Bacillus subtilis

FEBS Letters

Causey, Stuart¹  Ferrari, Eugenio¹  Olmos, Jorge²  Valle, Fernando²  Bollvar, Francisco²  Bolaños, Victor² 

[1] Genencor International Inc., 180 Kimball Way, South San Francisco, CA 94080, USA;Molecular Microbiology Department, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos 62271, Mexico

关键词: Sporulation;    abrB expression;    β-Galactosidase fusion;    Phosphorelay;    Transition state regulator;   

DOI  :  10.1016/0014-5793(96)00070-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The initiation of sporulation in Bacillus subtilis is under control of the transcriptional factor SpoOA. Most SpoOA mutants fail to initiate the sporulation process and all the sporulation initiated processes such as the synthesis of subtilisin. However, the product of spoOA9V, one of the several spoOA mutants characterized, distinguishes itself in the fact that, while it appears to effectively repress abrB, it fails to activate the spoIIA operon. The aim of this study was to examine the effect of the spoOA9V mutation on aprE expression and we found that in different genetic backgrounds, the spoOA9V mutation has a negative effect on aprE::lacZ expression.

【 授权许可】

Unknown