Phosphorylation and activation of hormone‐sensitive lipase in isolated macrophages

FEBS Letters

Small, Catherine A.²  Yeaman, Stephen J.²  Rogers, M.Perenna²  Goodacre, John A.¹ 

[1] Department of Rheumatology, Medical School, University of Newcastle-upon-Tyne, Newcastle-upon-Tyne NE2 4HH, UK;Department of Biochemistry & Genetics, Medical School, University of Newcastle-upon-Tyne, Newcastle-upon-Tyne NE2 4HH, UK

关键词: Hormone-sensitive lipase;    Protein phosphorylation;    Macrophage;    Cholesterol metabolism;    Enzymes: hormone-sensitive lipase or triacylglycerol lipase;    EC 3.1.1.3;    cyclic AMP-dependent protein kinase;    EC 2.7.1.37;   

DOI  :  10.1016/0014-5793(91)80179-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hormone-sensitive lipase (HSL) is responsible for the neutral cholesterol ester hydrolase activity in macrophages. Incubation of intact WEHI macrophages or mouse peritoneal macrophages leads to phosphorylation of HSL, which is increased by incubation with either dibutyryl cyclic AMP and 3-isobutyl-1-methylxanthine or okadaic acid. Correspondingly, these agents also activate neutral cholesterol ester hydrolase activity in intact WEHI cells. Regulation of mobilisation of esterified cholesterol in macrophages may be of antiatherogenic value, which this model system now allows us to investigate further.

【 授权许可】

Unknown