Phosphorylation of hormone‐sensitive lipase by cyclic GMP‐dependent protein kinase

FEBS Letters

Belfrage, Per¹  Strålfors, Peter¹ 

[1] Department of Physiological Chemistry 4, University of Lund, Box 94, S-221 00 Lund, Sweden

关键词: Cyclic GMP-dependent protein kinase;    Hormone-sensitive lipase;    Phosphorylotion site;    Cyclic AMP-dependent protein kinase;    Adipose tissue;   

DOI  :  10.1016/0014-5793(85)81086-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In intact rat adipocytes hormone-sensitive lipase has been shown to be phosphorylated on serine residues in two different phosphorylation sites: a regulatory site phosphorylated by cyclic AMP-dependent protein kinase and a basal site, which does not directly affect the enzyme activity, phosphorylated by cyclic AMP-independent protein kinase(s) [(1984) Proc. Natl. Acad. Sci USA 81, 3317-3321]. Cyclic GMP-dependent protein kinase catalyzed the phosphorylation of the same two phosphorylation sites on the isolated enzyme, at serine residues. Both sites were phosphorylated at about the same rate, with the hormone-sensitive lipase activity concomitantly enhanced.

【 授权许可】

Unknown