Primary structure of the site on bovine hormone‐sensitive lipase phosphorylated by cyclic AMP‐dependent protein kinase

FEBS Letters

Yeaman, Stephen J.²  Campbell, David G.¹  Cohen, Philip¹  Garton, Andrew J.² 

[1] MRC Protein Phosphorylation Group, Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland;Department of Biochemistry, University of Newcastle, Newcastle upon Tyne NE2 4HH, England

关键词: Hormone-sensitive lipase;    Protein phosphorylation;    cyclic AMP;    Amino acid sequence;    Protein kinase;    HSL;    hormone-sensitive lipase;    TFA;    trifluoroacetic acid;    Pth;    phenylthiohydantoin;   

DOI  :  10.1016/0014-5793(88)80799-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The primary structure of a region on hormone-sensitive lipase was determined to be: Lys-Thr-Glu-Pro-Met-Arg-Arg-Ser-Val-Ser-Glu-Ala-Ala-Leu-Thr-Gln-Pro-Glu-Gly-Pro-Leu-Gly-Thr-Asp-Ser-Leu-Lys. Ser-8 was the only residue in the intact protein phosphorylated by cyclic AMP-dependent protein kinase. However, Ser-10 also appeared to be present in a phosphorylated form, suggesting that it is a target for a distinct protein kinase in vivo.

【 授权许可】

Unknown