Inhibitory effect of calcium‐binding protein regucalcin on Ca2+‐activated DNA fragmentation in rat liver nuclei

FEBS Letters

Sakurai, Takashi¹  Yamaguchi, Masayoshi¹ 

[1] Department of Environmental Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 395 Yada, Shizuoka-City 422, Japan

关键词: Calcium;    Regucalcin;    DNA-fragmentation;    Rat liver nucleus;   

DOI  :  10.1016/0014-5793(91)80168-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incubation of isolated rat liver nuclei with ATP, NAD⁺, and micromolar Ca²⁺ concentrations of various metal ions resulted in extensive DNA hydrolysis. Half-maximal activity occurred with 1.0 μM Ca²⁺ added, and saturation of the process was observed with 10 μM Ca²⁺, The Ca²⁺ (10 μM)-activated DNA fragmentation was inhibited by the presence of Ca²⁺-binding protein regucalcin isolated from rat liver cytosol. The inhibitory effect of regucalcin was complete at 0.5 μM. At 25μM Ca²⁺ added, such an effect of regucalcin (1.0μM) was not seen, Regucalcin also inhibited Ca²⁺-activated DNA fragmentation in the presence or calmodulin (10 and 20 μg). The results show that regucalcin can inhibit the Ca²⁺-activated DNA fragmentation due to binding the metal, suggesting a role in regulation of liver nuclear functions.

【 授权许可】

Unknown