Expression of hepatic calcium‐binding protein regucalcin mRNA is mediated through Ca2+/calmodulin in rat liver

FEBS Letters

Shimokawa, Noriaki¹  Yamaguchi, Masayoshi¹ 

[1] Laboratory of Metabolism and Endocrinology, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka-city 422, Japan

关键词: Regucalcin;    Calcium;    Calmodulin;    Gene expression;    Northern blot analysis;    Rat liver;   

DOI  :  10.1016/0014-5793(93)81740-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of signal transduction-related factors was investigated to clarify the expression mechanism for mRNA of the hepatic Ca²⁺-binding protein regucalcin in the liver of rats. The change of regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin cDNA (0.6 kb). A single intraperitoneal administration of calcium chloride (15 mg Ca²⁺: 0.374 mmol/100 g body weight) to rats induced a remarkable increase of regucalcin mRNA in liver; the level was about 170% of controls at 30 min after administration. This increase was completely inhibited by simultaneous administration of trifluoperazine (5.0 mg/100 g), an antagonist of calmodulin. On the other hand, a single intraperitoneal administration of phorbol ester or dibutyryl cAMP (10–1,000 μg/100 g) did not cause a significant alteration of hepatic regucalcin mRNA levels. Also, administration of zinc, copper and cadmium (0.374 mmol of metal ion/100g) did not have an appreciable effect on hepatic regucalcin mRNA levels. These findings demonstrate that the expression of hepatic regucalcin mRNA is mediated through Ca²⁺/calmodulin.

【 授权许可】

Unknown