The oxygenation of cholesterol esters by the reticulocyte lipoxygenase

FEBS Letters

Wiesner, Rainer¹  Lankin, Vadim Z.²  Kühn, Hartmut¹  Belkner, Jutta¹ 

[1] Institute of Biochemistry, School of Medicine (Charitè), Humboldt University, Hessische Str. 3-4, O-1040 Berlin, Germany;All-Union Cardiological Research Center, Academy of Medical Sciences of the USSR, Moscow 12 1552, 3 Cherepkavskaja ul. 15A, USSR

关键词: Lipoxygenase;    Cholesterol ester;    Antherosclerosis;    SP-HPLC;    normal phase high-pressure liquid chromatography;    RP-HPLC;    reverse phase high-pressure liquid chromatography;    13S-HODE;    13S-hydroxy-9Z;    11E-octadecadienoic acid;    15S-HETE;    15S-hydroxy-5Z;    8Z;    11Z;    13E-eicosatetraenoic acid;    9-HODE;    9-hydroxy-10;    12-octadecadienoic acid;    13-HOTE;    13-hydroxy-9;    11;    15-octadecatrienoic acid;    GC/MS;    gas chromatography/mass spectrometry;    MSTFA;    N-methyl-N-trimethylsilyl-tri-fluoroacetamide;    DNBPG;    dinitrobenzoyl phenylglycine;    LDL;    low density lipoproteins;   

DOI  :  10.1016/0014-5793(91)80263-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The arachidonate 15-lipoxygenase from rabbit reticulocytes oxygenates cholesterol esters containing polyenoic fatty acids. Cholesterol esterified with saturated fatty acids is not oxygenated. The structures of the oxygenation products formed from various cholesterol esters have been identified by high pressure liquid chromatography, UV-spectroscopy and gas chromatography/mass spectroscopy. Oxygenated cholesterol esters have been detected in atherosclerotic plaques of human aortas.

【 授权许可】

Unknown