Requirement of monohydroperoxy fatty acids for the oxygenation of 15Ls‐HETE by reticulocyte lipoxygenase

FEBS Letters

Stender, H.¹  Lankin, V.Z.²  Schewe, T.¹  Kühn, H.¹  Rapoport, S.M.²  Nekrasov, A.²  Wiesner, R.¹ 

[1] Institute of Biochemistry, Humboldt University Berlin, Hessische Str. 3-4, DDR-104 Berlin, GDR;All Union Cardiological Research Centre, Academy of Medical Sciences of the USSR, 3 Cherepkowskaya ul. 15 A, Moscow 12 1552, USSR

关键词: Reticulocyte;    Lipoxygenase;    Enzyme activation;    Oxygenation;    15Ls-HETE;    15Ls-hydroxy-5;    8;    H;    13(Z;    Z;    Z;    E)-eicosatetraenoic acid;    13-HPODE;    13-hydroperoxy-9;    11-(Z;    E)-octadecadienoic acid;    GC/MS;    gas chromatography/mass spectroscopy;    SP-HPLC;    straight-phase high-pressure liquid chromatography;    CP-HPLC;    chiral-phase high-pressure liquid chromatography;    8;    15-DiHETE;    8-hydroxy-15-hydroxy5;    9;    11;    13-(Z;    E;    Z;    E)-eicosatetraenoic acid;    5;    15-DiHETE;    5-hydroxy-15-hydroxy-6;    8;    11;    13-(E;    Z;    Z;    E)-eicosatetraenoic acid;   

DOI  :  10.1016/0014-5793(86)80752-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The lipoxygenase from reticulocytes oxygenates 15L_s-HETE to 8-hydroperoxy-15-hydroxy-5,9,ll,13-eicosatetraenoic acid and 5-hydroperoxy-15-hydroxy-6,8,11,13-eicosatetraenoic acid only in the presence of catalytic concentrations of monohydroperoxy fatty acids. During this reaction the hydroperoxy fatty acids are converted to more polar products including hydroxy fatty acids. From kinetic measurements of 15L_sHETE oxygenation it was calculated that 1 mol monohydroperoxy fatty acid is consumed during the oxygenation of about 9 mol 15L_s-HETE.

【 授权许可】

Unknown