Formation of lipoxin B by the pure reticulocyte lipoxygenase

FEBS Letters

Stender, H.¹  Schewe, T.¹  Kühn, H.¹  Wiesner, R.¹  Alder, L.² 

[1] Institute of Biochemistry, Humboldt University Berlin, Hessische Str. 3-4 Berlin, GDR;Department of Chemistry, Humboldt University Berlin, Hessische Str. 1-2, DDR-104 Berlin, GDR

关键词: Lipoxygenase Multiple oxygenation Lipoxin;    15-H(P)ETE;    15-hydro(pero)xy-5;    8;    11;    13(Z;    Z;    Z;    E)-eicosatetraenoic acid;    5;    15-DiHETE;    5;    15 - dihydroxy - 6;    8;    11;    13(E;    Z;    Z;    E)-eicosatetraenoic acid;    RP-HPLC;    reversed-phase high-pressure liquid chromatography;    SP-HPLC;    straight-phase high-pressure liquid chromatography;    GC/MS;    gas chromatography/mass spectroscopy;   

DOI  :  10.1016/0014-5793(86)81026-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The pure reticulocyte lipoxygenase converts 5,15-DiHETE via a lipoxygenase reaction to 5,14,15-trihy-droxy-6, 8,10,12-eicosatetraenoic acid (a lipoxin B isomer) as shown by GC/MS analysis of its trimethylsilyl ether. With arachidonic acid, 15-HETE and 15-HETE methyl ester this lipoxin B isomer was also formed. The results presented here indicate that pure mammalian lipoxygenases are able to form lipoxins via sequential multiple oxygenation of arachidonic acid or its hydroxy derivatives.

【 授权许可】

Unknown