ATP‐dependent leukotriene export from mastocytoma cells

FEBS Letters

Ishikawa, Toshihisa¹  Schaub, Thomas¹  Keppler, Dietrich¹ 

[1] Division of Tumor Biochemistry, Deutsches Krebsforschungszentrum, D-6900 Heidelberg, Germany

关键词: ATP-dependent transport;    Mastocytoma cells;    Leukotriene export;    LT;    leukotriene;    LTE4NAc;    N-acetyl-leukotriene E4;    ω-OH-LTB4;    ω-hydroxy leukotriene B4;    ω-COOH-LTB4;    ω-carboxy leukotriene B4;    PG;    prostaglandin;    DNP-SG;    S-(2;    4-dinitrophenyl)-glutathione;    MK-886;    3-[1-(4-chlorobenzyl)-3-t-butyl-thio-5-isopropylindol-2-yl]-2;    2-dimethyl-propanoic acid;    MK-571;    3-(3-(2-(7-chloro-2-quinolinyl)ethenyl)phenyl)((S-dimethyl amino-3-oxopropyl)thio)propanoic acid;    LY245769;    (1S;    2R)-5-(3-[1-hydroxy-15;    15;    15-trifluoro-2-(2-1H-tetrazol-5-ylethylthio)-pentadeca-3(E);    5(Z)-dienyl]phenyl)-1H-tetrazole;   

DOI  :  10.1016/0014-5793(91)80256-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The biosynthesis of leukotrienes (LT) C₄ and B₄ is followed by an export of these mediators into the extracellular space. This transport was characterized using plasma membrane vesicles prepared from mastocytoma cells and identified as an ATP-dependent primary active process. The apparent K_m -values were 110 nM for LTC₄ and 48 μM for ATP. The transport rate was highest for LTC₄, whereas LTD₄, LTE₄, and N-acetyl-LTE₄ were transported with relative rates of 31, 12 and 8%, respectively, at a concentation of 10nM. LTB₄ transport was also dependent on ATP, LTC₄ transport with inhibited by LTD₄ receptor antagonists (IC₁₀ = 1.0 μM for MK-571 and 1.3 μM for LY245769) and by the inhibitor of leukotriene biosynthesis MK-886 (IC₁₀ = 1.8 μM). The ATP-dependent export carrier for leukotrienes in leukotriene-synthesizing cells represents a novel member of the family of ATP-dependent exit pumps.

【 授权许可】

Unknown