Evidence for leukotriene C4 transport mediated by an ATP‐dependent glutathione S‐conjugate carrier in rat heart and liver plasma membranes

FEBS Letters

Sogame, Yoshihisa²  Ishikawa, Toshihisa¹  Hayashi, Koichiro²  Kobayashi, Kazuo² 

[1] Department of Biochemistry, Osaka University Medical School, Osaka 530, Japan;Institute of Scientific and Industrial Research, Osaka University, Ibaraki 567, Japan

关键词: Leukotriene C4;    Glutathione S-conjugate;    ATP-dependent transport;    Plasma membrane;    (Rat heart;    rat liver);    LTC4;    leukotriene C4;    LTD4;    leukotriene D4;    LTE4;    leukotriene E4;    DNP-SG;    S-(2;    4-dinitrophenyl)-glutathione;    Hepes;    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;    4-hydroxynonenal;    trans-4-hydroxynon-2-enal;    SRS-A;    slow-reacting substance of anaphylaxis;    HPLC;    high-performance liquid chromatography;   

DOI  :  10.1016/0014-5793(89)81503-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using rat heart sarcolemma and liver plasma membrane vesicles, it has been verified that the transport of leukotriene C₄ (LTC₄) across membranes is an ATP-dependent process; the apparent K _m for LTC₄ was 150 nM (heart sarcolemma) or 250 nM (liver plasma membrane). S-(2,4-dinitrophenyl)-glutathione (DNP-SG) inhibited LTC₄ uptake into the vesicles dose-dependently (I ₅₀ = 25 (μM for both heart sarcolemma and liver plasma membrane vesicles). Mutual inhibition between LTC₄ and DNP-SG in uptake into the vesicles demonstrates that transport of LTC₄ is mediated by an ATP-dependent glutathione S-conjugate carrier.

【 授权许可】

Unknown