Leukotriene C4 inhibits ATP‐dependent transport of glutathione S‐conjugate across rat heart sarcolemma Implication for leukotriene C4 translocation mediated by glutathione S‐conjugate carrier

FEBS Letters

Ishikawa, Toshihisa¹ 

[1] Department of Biochemistry, Osaka University Medical School, Osaka 530, Japan

关键词: Leukotriene C4;    Glutathione S-conjugate;    ATP-dependent transport;    Sarcolemma;    (Rat heart);   

DOI  :  10.1016/0014-5793(89)80278-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sarcolemmal vesicles prepared from rat heart exhibited ATP-dependent uptake of S-(2,4-dinitrophenyl)glutathione (DNP-SG), which obeyed Michaelis-Menten kinetics with an apparent K _m of 21 μM for DNP-SG and a V _max of 0.27 nmol · 10 min⁻¹ · mg protein⁻¹. Several model glutathione S-conjugates inhibited DNP-SG uptake, but leukotriene C₄ inhibited uptake much more significantly even at lower concentrations (competitive inhibition, K _i = 1.5 μM). However, leukotrienes D₄ and E₄, which lack the γ-glutamyl moiety, were less effective. The results suggest that the ATP-dependent transport system has a high affinity for leukotriene C₄, and may be responsible for the translocation of this compound.

【 授权许可】

Unknown