Calcium‐permeable channels activated via guanine nucleotide‐dependent mechanism in human carcinoma cells

FEBS Letters

Kuryshev, Yury A.¹  Naumov, Alexander P.¹  Mozhayeva, Galina N.¹ 

[1] Institute of Cytology of Academy of Sciences of the USSR, 194064 Leningrad, USSR

关键词: Patch clamp;    Carcinoma cell;    Calcium channel;    Guanine nucleotide;    Epidermal growth factor;    EGF;    epidermal growth factor;    Hepes;    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;    EGTA;    ethylene glycol bis(β-aminoethyl ether)-N;    N;    N';    N'-tetraacetic acid;    GppNHp;    5'-guanylyl-imidodiphosphate;    GTP-γS;    5'-O-(3-thiotriphosphate);   

DOI  :  10.1016/0014-5793(90)80851-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Patch clamp experiments on human carcinoma A431 cells have revealed two types of Ca²⁺-permeable channels, the activity of which can be increased by the application of non-hydrolyzable analogues of GTP to the intracellular side of the membrane. With 105 mM Ca²⁺ in recording pipette at 30–33°C their unitary conductances (in pS) are 1.3 (SG-channels) and 2.4 (G-channels). G- and, possibly, SG-channels are activated from the extracellular side of the membrane with epidermal growth factor (EGF). The data are consistent with the hypothesis that both channels are activated via guanine nucleotide binding (G) proteins.

【 授权许可】

Unknown