Immunoelectron microscopical localization of the catalytic subunit of cAMP‐dependent protein kinases in brain microtubules and neurofilaments

FEBS Letters

Trinczek, Bernhard¹  Schwoch, Gerhild¹ 

[1] Abt. Klin. Biochemie, Zentrum Innere Medizin, Universität Göttingen, Robert-Koch-Str. 40, D-3400 Göttingen, Germany

关键词: Neurofilament;    Microtubule;    cAMP-dependent protein kinase;    Immunogold electron microscopy;    Rat brain;   

DOI  :  10.1016/0014-5793(90)80835-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The catalytic subunit of cAMP-dependent protein kinases was localized in microtubules and neurofilaments by immunogold electron microscopy. In microtubules, the label was similarly distributed as an immunolabel for the microtubule associated protein MAP 2. The neurofilaments showed no reaction with the MAP 2-antiserum. Our results support the suggestion of an in vivo role of cAMP-dependent protein kinases in the regulation of microtubules. In addition, this is the first demonstration that cAMP-dependent protein kinase is associated with neurofilaments.

【 授权许可】

Unknown