FEBS Letters | |
Interaction of smooth muscle caldesmon with phospholipids | |
Gusev, Nikolai B.1  Vorotnikov, Alexander V.1  | |
[1]Department of Biochemistry, School of Biology, Moscow State University, Moscow 119899, USSR | |
关键词: Caldesmon; Phospholipid; Phosphorylation; | |
DOI : 10.1016/0014-5793(90)80827-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
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【 摘 要 】
Taking into account the perimembrane localization of caldesmon [(1986) Nature 319, 68] and its ability to participate in the regulation of receptor clusterization [(1989) J. Biol. Chem. 264, 496], we studied the interaction of duck gizzard caldesmon with soybean phospholipids (azolectin). By using four independent methods, i.e. light scattering, gel-electrophoresis, gel-filtration and ultracentrifugation, we showed a Ca-independent complex formation between caldesmon and azolectin. Interacting with caldesmon, calmodulin is shown to dissociate the caldesmon-azolectin complex. It is supposed that the caldesmon-phospholipid interaction may affect caldesmon phosphorylation by Ca-phospholipid-dependent protein kinase. This effect may be important for various cell motility processes.
【 授权许可】
Unknown
【 预 览 】
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