Interaction of smooth muscle caldesmon with phospholipids

FEBS Letters

Gusev, Nikolai B.¹  Vorotnikov, Alexander V.¹ 

[1] Department of Biochemistry, School of Biology, Moscow State University, Moscow 119899, USSR

关键词: Caldesmon;    Phospholipid;    Phosphorylation;   

DOI  :  10.1016/0014-5793(90)80827-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Taking into account the perimembrane localization of caldesmon [(1986) Nature 319, 68] and its ability to participate in the regulation of receptor clusterization [(1989) J. Biol. Chem. 264, 496], we studied the interaction of duck gizzard caldesmon with soybean phospholipids (azolectin). By using four independent methods, i.e. light scattering, gel-electrophoresis, gel-filtration and ultracentrifugation, we showed a Ca-independent complex formation between caldesmon and azolectin. Interacting with caldesmon, calmodulin is shown to dissociate the caldesmon-azolectin complex. It is supposed that the caldesmon-phospholipid interaction may affect caldesmon phosphorylation by Ca-phospholipid-dependent protein kinase. This effect may be important for various cell motility processes.

【 授权许可】

Unknown