Electrophoretic separation by an improved method of fast myosin HCIIb‐,HCIId‐, and HCIIa‐based isomyosins with specific alkali light chain combinations

FEBS Letters

Pette, Dirk¹  Termin, Angelika¹ 

[1] Fakultät für Biologie, Universität Konstanz, Postfach 5560, D-7750 Konstanz, FRG

关键词: Myosin heavy chain;    Isomyosin electrophoresis;    Myosin heavy chain-based isomyosin;    Myosin alkali light chain;   

DOI  :  10.1016/0014-5793(90)81463-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An improved method of electrophoresis under nondenaturing conditions separated three electrophoretically distinct isomyosin triplets when applied to rat fast-twitch muscles displaying a predominance of one of the fast myosin heavy chain HCIIb, HCIId or HCIIa. The three isomyosin triplets, named FM1b-FM3b, FM1d-FM3d, FM1a-FM3a, correspond to the three possible alkali light chain (I.C.) combinations (L.C.??? homodimer, LC??? LC3f heterodimer, and LC3f homodimer) with each fast HC isoform. Different proportions of these various isomyosins suggested specific affinities of light chains LC??? and LC3f for the fast heavy chain isoforms.

【 授权许可】

Unknown