Identification of Myo3, a second type‐II myosin heavy chain in the fission yeast Schizosaccharomyces pombe

FEBS Letters

Yamamoto, Masayuki³  Mabuchi, Issei¹  Motegi, Fumio¹  Kitayama, Chikako²  Nakano, Kentaro³ 

[1] Division of Biology, Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153, Japan;Bionic Design Group, National Institute for Advanced Interdisciplinary Research, and Mechanical Engineering Laboratory, Tsukuba, Ibaraki, Japan;Graduate Program in Biochemistry and Biophysics, School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan

关键词: Fission yeast;    Cytokinesis;    Myosin heavy chain;    Septation;    F-actin ring;    DAPI;    4′;    6-diamidino-2-phenylindole;    Bodipy;    4;    4-difluoro-4-bora-3a;    4a-diaza-s-indacene;   

DOI  :  10.1016/S0014-5793(97)01510-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We cloned the myo3 ⁺ gene of Schizosaccharomyces pombe which encodes a type-II myosin heavy chain. myo3 null cells showed a defect in cytokinesis under certain conditions. Overproduction of Myo3 also showed a defect in cytokinesis. Double mutant analysis indicated that Myo3 genetically interacts with Cdc8 tropomyosin and actin. Myo3 may be implicated in cytokinesis and stabilization of F-actin cables. Moreover, the function of Myo2 can be replaced by overexpressed Myo3. We observed a modest synthetic interaction between Myo2 and Myo3. Thus, Myo2 and Myo3 seem to cooperate in the formation of the F-actin ring in S. pombe.

【 授权许可】

Unknown