Catecholamines inhibit steroidogenesis by cultured porcine thecal cells

FEBS Letters

Armstrong, David T.¹  Calaresu, Franco R.²  Morley, Paul² 

[1] Departments of Obstetrics and Gynaecology, The University of Western Ontario, London, Ont. N6A 5A5, Canada;Departments of Physiology, The University of Western Ontario, London, Ont. N6A 5A5, Canada

关键词: Porcine thecal cell;    Steroidogenesis;    Catecholamine;   

DOI  :  10.1016/0014-5793(90)81441-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ovaries of many species contain catecholamines and β-adrenergic receptors. The present studies were done to determine if catecholamines play a role in the regulation of androgen production by porcine theca cells. Basal and luteinizing hormone (LH)-stimulated androstenedione production was significantly inhibited by noradrenaline and isoproterenol. The inhibitory effects were dose-dependent and were enhanced when the cultures contained the carboxy-O-methyl transferase inhibitor, U-0521. The inhibitory effect of isoproterenol was reversed by the β-adrenergic antagonist, metoprolol. Isoproterenol caused a generalized inhibition of LH-stimulated steroidogenesis, decreasing the accumulation of pregnenolone, progesterone, androstenedione and estradiol in the culture medium. These studies suggest that catecholamines may be important regulators of thecal androgen production.

【 授权许可】

Unknown