Botulinum toxin types A, B and D inhibit catecholamine secretion from bovine adrenal medullary cells

FEBS Letters

Knight, D.E.¹ 

[1] MRC Secretory Mechanisms Group, Department of Physiology, King's College London, The Strand, London WC2 R2LS, England

关键词: (Adrenal medulla);    Botulinum toxin;    Exocytosis;    Ca2+;    Catecholamine;   

DOI  :  10.1016/0014-5793(86)81492-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Evoked catecholamine secretion from cultured bovine adrenal medullary cells is inhibited by commercially available botulinum toxins — types A, B and D (10⁴–10⁶ MLD/ml of culture medium). Basal secretion is also inhibited. The catecholamine content of such toxin-treated cells is larger than that of control cells and may in part be a result of the inhibition of basal release. The onset of action of botulinum toxin types A and D can be neutralised by their respective antisera. Concentrations of botulinum toxins A, B or D that inhibit secretion leave unaffected the ⁴⁵Ca²⁺ influxes normally associated with secretion. These data provide further evidence to support the idea [(1985) Nature 317, 719–721] that botulinum toxins block secretion by acting downstream of the Ca²⁺ transient at or near the site of exocytosis.

【 授权许可】

Unknown