FEBS Letters | |
Botulinum toxin types A, B and D inhibit catecholamine secretion from bovine adrenal medullary cells | |
Knight, D.E.1  | |
[1] MRC Secretory Mechanisms Group, Department of Physiology, King's College London, The Strand, London WC2 R2LS, England | |
关键词: (Adrenal medulla); Botulinum toxin; Exocytosis; Ca2+; Catecholamine; | |
DOI : 10.1016/0014-5793(86)81492-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Evoked catecholamine secretion from cultured bovine adrenal medullary cells is inhibited by commercially available botulinum toxins — types A, B and D (104–106 MLD/ml of culture medium). Basal secretion is also inhibited. The catecholamine content of such toxin-treated cells is larger than that of control cells and may in part be a result of the inhibition of basal release. The onset of action of botulinum toxin types A and D can be neutralised by their respective antisera. Concentrations of botulinum toxins A, B or D that inhibit secretion leave unaffected the 45Ca2+ influxes normally associated with secretion. These data provide further evidence to support the idea [(1985) Nature 317, 719–721] that botulinum toxins block secretion by acting downstream of the Ca2+ transient at or near the site of exocytosis.
【 授权许可】
Unknown
【 预 览 】
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