Ca2+‐dependent cosecretion of adrenomedullin and catecholamines mediated by nicotinic receptors in bovine cultured adrenal medullary cells

FEBS Letters

Wada, Akihiko¹  Ichiki, Yoshinari¹  Eto, Tanenao¹  Yamamoto, Yoshitaka¹  Kangawa, Kenji²  Niina, Hiromi³  Katoha, Fumi¹  Kitamura, Kazuo¹ 

[1] First Department of Internal Medicine, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-16, Japan;National Cardiovascular Center Research Institute, Fujishirodai, Suita, Osaka 565, Japan;Department of Pharmacology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-16, Japan

关键词: Adrenomedullin;    Catecholamine;    Secretion;    Ca2+;    Nicotinic receptor;    Adrenal medulla;    ir-AM;    immunoreactive adrenomedullin;    CGRP;    calcitonin gene-related peptide;    ANP;    atrial natriuretic peptide;    TFA;    trifluoroacetic acid;    EDTA;    ethylenediaminetetraacetic acid;    HPLC;    high-performance liquid chromatography;    KRP;    Krebs-Ringer phosphate;    EC50;    half-maximal effective concentration;   

DOI  :  10.1016/0014-5793(94)00566-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bovine cultured adrenal medullary cells (4 × 10⁶) contained 4266.5 ± 370.0 fmol of immunoreactive adrenomedullin and 373.4 ± 32.6 mnol of catecholamines. Nicotinic (but not muscarinic) receptors mediated the Ca²⁺-dependent co-secretion of adrenomedullin and catecholamines, with the molar ratio of adrenomedullin/catecholamines secreted into the medium being equal to the ratio stored in the cells. The concentration-response curve of carbachol for adrenomedullin secretion (EC₅₀ 42 μM) was similar to that for catecholamine secretion (EC₅₀ 63 μM). Reverse phase HPLC analysis showed that immunoreactive adrenomedullins in the cells and secreted into the medium were both eluted exclusively at the position almost identical to synthetic human adrenomedullin[1–52]NH₂.

【 授权许可】

Unknown