Regulation of cytochrome P‐450 CYPIA1 gene expression and protooncogene expression by growth factors in primary hepatocytes

FEBS Letters

Taniguchi, Hisaaki¹  Höhne, Martin²  Kahl, Georg F.²  Becker-Rabbenstein, Volker² 

[1] Institute of Experimental Pathology, German Cancer Research Center, D6900 Heidelberg, FRG;Institute of Pharmacology and Toxicology, University of Göttingen, D3400 Göttingen, FRG

关键词: Hepatocyte;    Growth factor;    Cytochrome P-450 gene regulation;    EGF;    epidermal growth factor;    TGFα;    transforming growth factor α;    3-MC;    3-methylcholanthrene;    CYP;    cytochrome P-450;    EROD;    7-ethoxyresorufin-O-deethylase;   

DOI  :  10.1016/0014-5793(90)81089-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of growth factors on the cytochrome P-450 (CYPIA1) gene expression was studied in primary mouse hepatocytes. Of the three growth factors used, i.e. epidermal growth factor (EGF), transforming growth factor α (TGFα) and insulin, only EGF or TGFα completely blocked CYPIA1 expression in the presence of the CYPIA1 inducer 3-methylcholanthrene (3-MC). This repression was not linked to cell cycle progression of the hepatocyte because insulin was active to induce ‘early immediate genes’ and DNA replication as well as EGF/TGFα but failed to suppress CYPIA1 expression. A specific EGF/TGFα receptor-mediated function may repress CYPIA1 gene expression and contribute to the acquisition of a xenobiotic drug resistance phenotype.

【 授权许可】

Unknown