A genomic clone encoding a cryptophyte phycoerythrin α‐subunit

FEBS Letters

Hiller, Roger G.²  Jenkins, Jon²  Godovac-Zimmermann, Jasminka³  Speirs, Jim¹ 

[1] CSIRO Division of Horticulture, North Ryde, N.S.W. 2113, Australia;School of Biological Sciences, Macquarie University, N.S.W. 2109, Australia;John Curtin School of Medical Research, Australian Natlonal University, Canberra, A.C.T. 2600, Australia

关键词: Phycoerythrin;    α-Subunit;    Amino acid sequence;    Genomic clone;    Signal sequence;    Chroomonas CS24;    Cryptophyceae;   

DOI  :  10.1016/0014-5793(90)81082-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A genomic library of Chroomonas (Cryptophyceae) DNA has been constructed in λEMBL4. Using a synthetic oligomer as a hybridization probe, a clone containing a phycoerythrin α-subunit has been obtained and sequenced. The principal α₁ and α₂-subunits of the holoprotein have been partially sequenced by sequential Edman degradation and differ from the DNA derived sequence, providing evidence for at least 3 α-subunit genes. The nucleotide sequence of the α-subunit gene is GC rich and encodes an N-terminal extension which is putatively thylakoid-lumen directing.

【 授权许可】

Unknown