Cloning and characterization of the human gene for the α‐subunit of Gi2, a GTP‐binding signal transduction protein

FEBS Letters

Spiegel, A.M.¹  Carter, A.D.¹  Weinstein, L.S.¹ 

[1] Molecular Pathophysiology Section, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA

关键词: G-protein;    Signal transduction;    Genomic clone;    GC box;   

DOI  :  10.1016/0014-5793(88)80764-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We isolated and characterized human genomic clones encompassing the gene for the α-subunit of G_i2, a GTP-binding signal transduction protein abundantly expressed in myeloid cells. The gene is divided into 9 exons and spans 23.5 kb. Exons 2, 6 and 7 encode putative guanine nucleotide-binding domains that are highly conserved among GTP-binding proteins. A polyadenylation signal located within exon 9 predicts an MRNA size (∼ 2.3 kb) several hundred bases longer than that of published cDNAs, and consistent with the size seen on RNA blot hybridization. Primer extension and S₁ nuclease analysis determined a major and several minor transcriptional start sites. The first exon and 5′ flanking region are highly G+ C rich, contain several GC boxes (SP1 transcription factor binding sites), a CAAT box, and lack a TATA box. The presumptive promoter region is thus similar to that of ras and other widely expressed genes.

【 授权许可】

Unknown