Protein phosphorylation in isolated nuclei from etiolated Avena seedlings Effects of red/far‐red light and cholera toxin

FEBS Letters

Biswal, Basanti¹  Romero, Luis C.¹  Song, Pill-Soon¹ 

[1] Institute of Cellular and Molecular Photobiology and Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA

关键词: Signal transduction;    G-protein;    Phytochrome;    Nuclei;    Avena saliva;    Cab;    chlorophyll a/b binding protein gene;    GTP-binding proteins or G-proteins;    guanine nucleotide binding proteins;    GDPβS;    guanosine 5′-O-(2-thiodiphosphate);    phy;    phytochrome gene;   

DOI  :  10.1016/0014-5793(91)80510-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the phosphorylation/dephosphorylation of several nuclear proteins in isolated nuclei from etiolated Avena seedlings as a function of red/far-red light. The effect of stimulatory (ADP-ribosylation by cholera toxin) or inhibitory (GDPβS) conditions for GTP-binding proteins was also studied. Red or far-red light enhanced the phosphorylation level of 2 nuclear proteins with molecular masses of 75 and 60 kDa. The phosphorylation pattern was affected by the addition of cholera toxin or GDPβS to the isolated nuclei. At least 2 proteins with molecular masses of 24 and 75 kDa cross-reacted by Westerrs blot with GTP-binding protein antibodies.

【 授权许可】

Unknown