Premature termination of transcription and alternative splicing in the human transacylase (E2) gene of the branched‐chain α‐ketoacid dehydrogenase complex

FEBS Letters

Lee, Jun²  Chuang, David T.²  Cox, Rody P.¹  Fisher, Charles W.²  Lau, Kim S.² 

[1] Department of Internal Medicine, The University of Texas Southwestern Medical Center, Dallas, TX 75235-9038, USA;Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, TX 75235-9038, USA

关键词: Genomic clone;    cDNA;    E2 gene;    Alternative splicing;   

DOI  :  10.1016/0014-5793(91)80155-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated a human genomic clone hgE2-14 containing exons 5,6,7 and 8 of the branched-chain α-ketoacid dehydrogenase E2 transacylase gene. Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence between nucleotides 938 and 1521. We have identified consensus splice site junctions flanking exon 8 and also a cryptic 3??? splice site 370 bases upstream from the start of exon 8 in the gene. In addition, two polyadenylation signals located in the hE2-1 cDNA are also present in the intronic sequence downstream of exon 8 which promote termination of transcription. The data indicate that shortened human liver E2 transcripts undergo alternative splicing to yield mRNA of the hE2-1 type.

【 授权许可】

Unknown